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目的：制备抗小鼠白细胞介素２受体（ＩＬ－２Ｒ）单克隆抗体（ＭｃＡｂ）。方法：利用杂交瘤技术获得杂交瘤细胞株，并用细胞ＥＬＩＳＡ方法筛选阳性克隆。结果：得到３株分泌抗小鼠ＩＬ－２ＲＭｃＡｂ的杂交瘤细胞株，其中８Ｃ８对ＣｏｎＡ刺激淋转有抑制作用，且ｓＩＬ－２Ｒ可抑制８Ｃ８与ＩＬ－２Ｒα阳性细胞的结合。结论：８Ｃ８是抗小鼠ＩＬ－２Ｒα抗体。
Objective: To prepare anti-mouse interleukin-2 receptor monoclonal antibody (McAb). Methods: The hybridoma cell lines were obtained by hybridoma technique and the positive clones were screened by ELISA. RESULTS: Three hybridoma cell lines secreting anti-mouse IL-2RMcAb were obtained. 8C8 inhibited the lymph node metastasis of ConA and sIL-2R inhibited the binding of 8C8 to IL-2Rα-positive cells. Conclusion: 8C8 is an anti-mouse IL-2Rα antibody.