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目的研究表绿茶活性成分没食子儿茶素-3-没食子酸酯(epigallocatechin gallate,EGCG)对大鼠肾脏缺血再灌注损伤是否具有保护作用。方法将12只Lewis大鼠随机分为2组:(1)实验组大鼠7只,于肾缺血后的再灌注期将10 mg/kg的EGCG置于2ml生理盐水中静脉注射;(2)对照组大鼠5只,于肾缺血后的再灌注期静脉注射2 ml生理盐水。2 h后处死动物,收集血液和肾脏标本,行血肌酐(Cr)、尿素氮(BUN)、肾组织超氧化物歧化酶(SOD)和脂质过氧化产物丙二醛(MDA)含量测定,并进行组织病理和超微病理检查。结果实验组大鼠血Cr和BUN水平明显低于对照组(P<0.05),肾组织中SOD活性较对照组明显升高(P<0.01),肾组织脂质过氧化产物MDA的含量较对照组明显降低(P<0.01)。病理学检测表明实验组大鼠肾小管周围毛细血管内未见淤血,肾小管上皮细胞变性及线粒体的损伤减轻。结论EGCG对大鼠肾脏缺血再灌注损伤具有保护作用。
Objective To investigate whether epigallocatechin gallate (EGCG), a green tea active ingredient, has a protective effect on kidney ischemia-reperfusion injury in rats. Methods Twelve Lewis rats were randomly divided into two groups: (1) Seven rats in the experimental group were injected with 10 mg / kg EGCG in 2 ml normal saline during the reperfusion period after renal ischemia; (2) ) Control rats 5 rats, intravenous injection of 2 ml of saline in the reperfusion period after renal ischemia. After 2 hours, the animals were sacrificed and the blood and kidneys were collected for serum creatinine (Cr), blood urea nitrogen (BUN), superoxide dismutase (SOD) and malondialdehyde (MDA) And histopathological and ultrastructural examination. Results The level of serum Cr and BUN in the experimental group was significantly lower than that in the control group (P <0.05). The SOD activity in the kidney tissue was significantly higher than that in the control group (P <0.01) Group was significantly lower (P <0.01). Pathological examination showed that there was no congestion in the capillaries around the renal tubules of rats in the experimental group, and the degeneration of renal tubular epithelial cells and mitochondrial damage were alleviated. Conclusion EGCG has a protective effect on renal ischemia-reperfusion injury in rats.