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目的研究有机碘(DIT)和无机碘(KI)对体外培养的人甲状腺细胞(TEC)分泌三碘甲腺原氨酸(T3)、甲状腺素(T4)及甲状腺球蛋白(Tg)功能的影响。方法取正常甲状腺细胞进行原代细胞培养,将细胞密度调整为2×105/ml,加入DIT和KI溶液(含碘最终浓度分别为0、10-8、10-7、10-6、10-5、10-4、10-3 mol/L),培养48 h。采用放射免疫法测定细胞培养上清液中T3、T4及Tg的含量。结果与对照组比较,各剂量DIT组和KI组甲状腺细胞分泌的T3、T4和Tg含量均较高,差异有统计学意义(P<0.01)。当KI和DIT浓度均为10-5 mol/L时,T3含量升至最高;当KI浓度为10-5 mol/L或DIT浓度为10-4 mol/L时,T4含量升至最高;当KI和DIT浓度均为10-6 mol/L时,Tg含量升至最高。与相同剂量KI组比较,DIT浓度为10-6 mol/L时的T3含量较低,DIT浓度为10-5和10-4 mol/L时T4含量较低,DIT浓度为10-6和10-3 mol/L时的Tg含量均较低,差异有统计学意义(P<0.05或P<0.01)。结论适量碘可促进甲状腺细胞分泌T3和T4及Tg,DIT对甲状腺激素分泌的影响程度稍低于KI。
Objective To investigate the effects of organic iodine (DIT) and inorganic iodine (I) on the secretion of triiodothyronine (T3), thyroxine (T4) and thyroglobulin (Tg) in cultured human thyrocytes . Methods Normal thyroid cells were cultured in primary cells, the cell density was adjusted to 2 × 105 / ml, DIT and KI solutions were added (the final concentrations of iodine were 0,10-8,10-7,10-6,10- 5,10-4,10-3 mol / L) and cultured for 48 h. Radioimmunoassay was used to determine the content of T3, T4 and Tg in cell culture supernatant. Results Compared with the control group, the levels of T3, T4 and Tg secreted by thyroid cells in DIT group and KI group were significantly higher than those in control group (P <0.01). When the concentrations of KI and DIT were both 10-5 mol / L, the content of T3 rose to the highest. When the concentration of KI was 10-5 mol / L or the concentration of DIT was 10-4 mol / L, the content of T4 rose to the highest. When the concentrations of KI and DIT were both 10-6 mol / L, the content of Tg increased to the highest. Compared with the same dose of KI group, the content of T3 was lower when the DIT concentration was 10-6 mol / L, the content of T4 was lower when the DIT concentration was 10-5 and 10-4 mol / L, the DIT concentration was 10-6 and 10 The Tg of -3 mol / L was lower than that of control (P <0.05 or P <0.01). Conclusion Iodine can promote the secretion of T3 and T4 and Tg of thyrocytes. The effect of DIT on thyroid hormone secretion is slightly lower than that of KI.