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目的:从血清总超氧化物歧化酶(T-SOD)、一氧化氮(NO)、丙二醛(MDA)含量和肝、脂肪组织病理学改变角度探讨加味温胆汤抗大鼠营养性肥胖的机制。方法:采用“髙脂乳剂+普通饲料”双重喂养法复制大鼠营养性肥胖模型。雄性断乳SD大鼠30只,按体重均匀分为空白对照组、模型对照组和加味温胆汤组,每组10只。各组动物每天均喂饲普通饲料,除空白对照组外,其余组动物每天上午灌胃给予自制高脂乳剂20 mL·kg-1造模,连续4周。从第5周开始,于每天下午灌胃给予实验组加味温胆汤(7.74 g·kg-1),空白对照组与模型对照组给同体积水,连续4周。末次给药后,禁食不禁水12 h,取血清检测T-SOD,NO,MDA水平;分离肝、脂肪组织,10%甲醛溶液固定,常规制片,HE染色,光镜下观察肝、脂肪细胞的病变。结果:加味温胆汤组大鼠血清NO含量明显低于模型组(P<0.01);大鼠肝细胞界限清楚,排列整齐,脂变肝细胞散在;脂肪细胞数量增多,体积减小。结论:加味温胆汤抗大鼠营养性肥胖的机制可能与降低血清NO水平、对抗肝细胞脂肪变性、缩小脂肪细胞体积等有关。
OBJECTIVE: To investigate the effects of Jiawei Wendan decoction on rat nutritional obesity from the perspectives of serum total superoxide dismutase (T-SOD), nitric oxide (NO), malondialdehyde (MDA) and hepatic and adipose tissue histopathological changes Mechanisms. Methods: The rat model of nutritional obesity was reproduced by using “Fat emulsion + normal feed” double feeding method. Thirty male weanling SD rats were equally divided into blank control group, model control group and Jiawei Wendan Decoction group according to body weight, with 10 rats in each group. Each group of animals were fed ordinary feed everyday, except the blank control group, the other animals were orally given daily high fat emulsion 20 mL · kg-1 orally for 4 weeks. From the fifth week onwards, Jiawei Wendan Decoction (7.74 g · kg-1) was given to the experimental group orally every afternoon. The blank control group and the model control group were given the same volume of water for 4 weeks. After the last administration, the rats were fasted for 12 h. The levels of T-SOD, NO and MDA were determined by serum. Liver and adipose tissue were isolated and fixed in 10% formaldehyde solution. Cell lesions. Results: The content of serum NO in Jiawei Wendan Decoction group was significantly lower than that in model group (P <0.01). The rat hepatocytes had clear boundary and neat arrangement, and scattered hepatocytes were scattered. The number of adipocytes increased and the volume decreased. Conclusion: The mechanism of Jiawei Wendan Decoction on rat nutritional obesity may be related to the reduction of serum NO level, the antagonism of fatty degeneration of hepatocytes and the reduction of the volume of adipocytes.