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目的观察分析内异消症汤治疗子宫内膜异位症对于在位、异位内膜间质细胞分泌功能的影响特点。方法选取30只健康的SD雌性大鼠,随机分为内异消症汤组、米非司酮组与空白组,体外分离在位、异位内膜间质细胞并原代培养,免疫细胞化学法鉴定分析,制备大鼠血清,米非司酮组及内异消症汤组分别加入对应药物,MTT法对血清间质细胞生长情况进行测定、ELISA技术测定间质细胞Ang-2分泌情况。结果内异消症汤组在位内膜间质细胞生长OD值(0.083±0.005)、异位内膜间质细胞生长OD值(0.056±0.004),生长OD值明显低于空白组和米非司酮组(P<0.05)。内异消症汤组在位内膜间质细胞Ang-2分泌(10.902±0.056)ng/ml、异位内膜间质细胞Ang-2分泌(20.583±0.057)ng/ml,分泌明显少于空白组和米非司酮组(P<0.05)。结论内异消症汤治疗子宫内膜异位症可有效防控间质细胞增殖并一直细胞分泌Ang-2,从根本上达到治疗目的。
Objective To observe the effect of Nei Yi Xiao Tang on endometriosis in eutopic and ectopic endometrial stromal cells. Methods Thirty healthy female Sprague-Dawley rats were randomly divided into two groups: Neiwei Xiaozheng Decoction group, mifepristone group and blank group. The eutopic and ectopic endometrial stromal cells were isolated and cultured in vitro. Immunocytochemistry The method of forensic analysis, preparation of rat serum, mifepristone group and Neiwai Xiaoyan Decoction group were added corresponding drugs, MTT assay of serum stromal cell growth was measured, ELISA assay stromal cells Ang-2 secretion. Results The OD value of eutopic endometrial stromal cells (0.083 ± 0.005) in euthanasia group and OD value of eutopic endometrial stromal cells (0.056 ± 0.004) were significantly lower than those in control group Ketone group (P <0.05). The secretion of Ang-2 (10.902 ± 0.056) ng / ml in eutopic endometrial stromal cells and the secretion of Ang-2 in ectopic endometrial stromal cells (20.583 ± 0.057) ng / ml were significantly lower Blank group and mifepristone group (P <0.05). Conclusion Nei Yi Xiao Tang Decoction treatment of endometriosis can effectively prevent and control interstitial cell proliferation and secretion of Ang-2 cells, and ultimately achieve the purpose of treatment.