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恶性肿瘤细胞与植入前小鼠胚胎的共培养是研究两者在共同环境中生物学行为变化的重要手段。取得小鼠交配后3.5d囊胚,分别与鼠源性肿瘤细胞株B16和人源性肿瘤细胞株HepG2共培养,观察胚胎的发育状况以及肿瘤细胞增殖、凋亡变化。结果发现胚胎在体外与肿瘤细胞共存的环境中能正常发育,且与对照相比其脱带率、贴壁率和扩展率均有显著提高(P<0.05),但肿瘤细胞在增殖、凋亡等生物学行为方面无明显变化(P>0.05)。实验表明早期胚胎和肿瘤细胞可以在共同的微环境中正常生长,胚胎和肿瘤细胞对生存条件的选择具有相似性;肿瘤细胞提供的生长环境可以促进胚胎的发育,这可能与肿瘤细胞提供的适宜胚胎发育的环境和两者在基因表达、生长因子及信号转导机制等生物学方面的相似性有关。
Co-culture of malignant tumor cells with preimplantation mouse embryos is an important means to study the biological behavior change between the two in a common environment. The blastocysts were obtained 3.5 days after mating, and were respectively co-cultured with murine tumor cell line B16 and human tumor cell line HepG2 to observe the embryonic development and the changes of tumor cell proliferation and apoptosis. The results showed that the embryos could develop normally in the environment coexisting with tumor cells in vitro, and the rates of de-attachment, adherent and expansion were significantly increased compared with the control (P <0.05), but the proliferation and apoptosis of tumor cells No significant changes in other biological behaviors (P> 0.05). Experiments show that early embryos and tumor cells can grow normally in a common microenvironment, and embryos and tumor cells have similar selection of living conditions. The growth environment provided by tumor cells can promote the development of the embryo, which may be compatible with the appropriate The environment of embryonic development is related to the biological similarity between the two genes, such as gene expression, growth factors and signal transduction mechanisms.