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目的探讨Toll样受体(TLR)和细胞因子在细菌性痢疾(以下简称“菌痢”)患儿免疫应答中的作用,为该病的防治提供理论线索。方法研究对象为确诊菌痢的患儿55例,建立荧光定量PCR方法对外周血单个核细胞5种TLR和6种炎性细胞因子的基因表达进行定量检测。结果与正常对照组比较,发病在3 d以内和3 d以后的小儿外周血白细胞中TLR2、TLR4 mRNA的表达水平均有明显的升高。IL-8 mRNA的表达水平在发病的全程比对照组均明显升高,IL-12 p40 mRNA的表达水平在起病早期有3倍的明显升高。升高的TLR2、TLR4和升高的炎性细胞因子之间呈明显的正相关关系。结论菌痢患儿TLR2和TLR4显著升高,并通过促使细胞因子的产生启动并参与免疫应答。
Objective To investigate the role of Toll-like receptors (TLRs) and cytokines in immune response in children with bacillary dysentery (hereinafter referred to as “bacillary dysentery”), and to provide theoretical clues for the prevention and treatment of this disease. Methods Fifty-five children diagnosed with bacillary dysentery were included in the study. Quantitative PCR was used to detect the expression of five TLRs and six inflammatory cytokines in peripheral blood mononuclear cells. Results Compared with the normal control group, the expression levels of TLR2 and TLR4 mRNA in peripheral blood leukocytes were significantly increased within 3 days and 3 days after onset. The expression level of IL-8 mRNA in the whole course of the disease was significantly higher than that in the control group, and the expression level of IL-12 p40 mRNA was significantly increased 3-fold in the early stage of onset. Elevated TLR2, TLR4 and elevated inflammatory cytokines showed a significant positive correlation between. Conclusion In children with bacillary dysentery, TLR2 and TLR4 are significantly increased, and they are initiated and involved in immune response by promoting the production of cytokines.