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研究了配位聚合物锇 -聚乙烯吲哚 [Os( bpy) 2 ( PVI) 1 0 Cl]Cl和 Nafion双层膜修饰玻碳电极的电化学特性 ,该膜对肾上腺素 ( EP)的电化学氧化有催化作用 ,在通常的生理条件下 ( p H7.0 ) ,催化电流与 EP浓度在1 .0× 1 0 - 6～ 8.6× 1 0 - 5 mol/L范围内呈良好的线性关系 ,相关系数为 0 .9987.Nafion膜排除了抗坏血酸( AA)的干扰 ,表现出较高的灵敏度、选择性及良好的稳定性 .该电极可在 +2 50 m V下进行 EP的安培法测定 .用旋转圆盘电极对电催化过程的动力学进行了研究 ,催化速率常数 kch为 3 .53× 1 0 3mol- 1 · L· s- 1 .在较高 EP浓度下 ,催化电流与浓度的关系表现出 Michaelis-Menten型响应 ,Michaelis-Menten常数 Km 为1 .4 7mmol/L.
The electrochemical properties of coordination polymer Osmium - poly (vinyl indole) [Os (bpy) 2 (PVI) 1 0 Cl] Cl and Nafion double - layer modified glassy carbon electrode were studied. Under the normal physiological conditions (p H7.0), the catalytic current showed a good linear relationship with the concentration of EP in the range of 1 × 10 6 -6 6 × 10 5 mol / L , With a correlation coefficient of 0.9987. Nafion membrane excludes the interference of ascorbic acid (AA), showing higher sensitivity, selectivity and good stability. The electrode can be used in the amperometric determination of EP at +2 50 mV The kinetics of the electrocatalytic process was investigated using a rotating disk electrode at a kinetic rate constant of 3.53 × 10 3 mol -1 · L · s -1. At higher EP concentrations, the catalytic current and concentration The relationship shows a Michaelis-Menten-type response with a Michaelis-Menten constant Km of 1.47 mmol / L.