据报道P物质(Substance P,SP)是伤害性初级传入末梢释放的一种兴奋性递质,在痛觉调制过程中,脊髓内SP起着传递痛觉信息和镇痛的双重作用,但这一结论多数是依据注入外源性SP的结果,至于内源性SP在镇痛与致痛中所起的作用,十分复杂,机理尚不清楚.本文应用对神经元功能活动进行细胞定位的c-fos技术,研究SP对伤害性刺激(Formalin,福尔马林,F)所激活的脊髓神经元的影响,进一步了解脊髓内P物质在痛觉调制中所起的作用. It has been reported that Substance P (SP) is an excitatory neurotransmitter released from nociceptive primary afferent terminals. SP plays a dual role in the process of pain modulation in transmitting pain information and analgesia during pain modulation. However, Most of the conclusions are based on the results of the injection of exogenous SP, as endogenous SP in the analgesic and induced pain, is very complicated, the mechanism is not clear.In this paper, the neuronal functional activity of cellular localization c- fos technology to study the effects of SP on spinal cord neurons activated by Formalin (formalin, F) and to further understand the role of substance P in spinal modulation in the regulation of pain.