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目的:利用胃癌及癌前病变生物学恶性信息，检测胃粘膜活检组织中幽门弯曲杆菌感染与P21、P53表达的关系。方法：收集胃粘膜活检标本，应用免疫组化技术，常规石蜡切片、HE、Giemsa染色观察。结果：幽门弯曲杆菌（HP）感染率：慢性浅表性胃炎（CSG）30．8％（8／26），慢性萎缩性胃炎67.9％19／28），肠上皮化生63．6％（21／23），不典型增生（AH）73％（22／30），胃癌（GC）64．1％（25／39）。HP感染与各型病变中P21表达阳性率（％）关系：HP阳性者为36.8～64．0，其中GC为最高，AH为次；HP阴性者为22．2～57．1，与P53表达阳性率（％）关系：HP阳性者为31.6～60．0，其中GC为最高，AH为次；HP阴性者为11．1～50．0。结论：对胃癌及癌前病变进行HP及两种蛋白检测，有助于认识HP感染是胃癌发生的高危因素及胃粘膜萎缩→肠化生→异型增生→癌变的P21、P53表达阳性率呈递增的规律。
OBJECTIVE: To use the malignant biological information of gastric cancer and precancerous lesions to detect the relationship between Campylobacter pylori infection and the expression of P21 and P53 in gastric mucosa biopsy. METHODS: Gastric mucosal biopsy specimens were collected, immunohistochemical techniques, paraffin sections, HE, and Giemsa staining were performed. RESULTS: Campylobacter (HP) infection rates were chronic superficial gastritis (CSG) 30.8% (8/26), chronic atrophic gastritis 67.9% 19/28), and intestinal metaplasia 63.6% (21 /23), Atypical hyperplasia (AH) 73% (22/30), Gastric cancer (GC) 64.1% (25/39). Relationship between HP infection and the positive rate of P21 expression in various types of lesions (HP): 36.8 to 64.0, with GC being the highest and AH being the second; HP being negative, 22.2 to 57.1, and P53 expression. Positive rate (%) relationship: HP positive was 31.6 to 60.0, of which GC was the highest, AH was the second; HP negative was 11.1 to 50.0. Conclusion: Detection of HP and two proteins in gastric cancer and precancerous lesions helps to understand that HP infection is a high risk factor for gastric cancer and the positive rate of P21, P53 expression in gastric mucosal atrophy → intestinal metaplasia → dysplasia → canceration is increasing The law.