作者从大白鼠和小鼠的胎儿移植背根神经节和整个横断面(肌原节、索和神经节)之器官型培养物,并采用Maximow双盖片培养法培养之。所用培养基系由Eagle氏最低基础培养基(MEM)、牛血清超滤物、人胎盘血清和鸡胚浸液各按等量相加而成。并在每100毫升中再补加600毫克葡萄糖。移植于盖片的上述培养物,用一滴培养基喂养之,在35℃培养,并每周在Simm氏平衡盐水内洗二次,洗后再用一滴新鲜培养基喂养。待培养物成熟 The authors transplanted dorsal root ganglia and organotypic cultures of entire cross-sections (myotubes, cords and ganglia) from the fetus of mice and mice and cultured using a Maximow double-lid culture. Culture medium used by Eagle’s Minimum Basal Medium (MEM), bovine serum ultrafiltration, human placental serum and chicken embryo extract by the same amount of each added. And add 600 mg of glucose to every 100 ml. The above cultures, transplanted onto the coverslip, were fed in a drop of culture medium, incubated at 35 ° C, and washed twice weekly in Simm’s balanced saline before being washed with a drop of fresh medium. Let the culture mature