Purpose: To estimate the random measurement error in visual acuity (VA) determ ination in the clinical environment in cataractous, pseudophakic and healthy eye s. Methods: The VAs of patients referred for cataract surgery or consultation by ophthalmic professionals were re-examined and the VA results for distance usin g projector acuity charts were compared. Refractive errors were also remeasured. A total of 99 eyes (41 cataractous, 36 pseudophakic and 22 healthy eyes) were e xamined. The healthy comparison group consisted of hospital staff. Only one eye of each person and eyes with Snellen VAs of 0.3-1.3 (logMAR 0.52 to-0.11) were included. The mean time interval between the first and second examinations was 45 days. Results: The estimated standard deviation of measurement error (SDME) o f repeated VA measurements of all eyes was logMAR 0.06. Eyes with the lowest VA (0.3-0.45) had the largest variability (SDME logMAR 0.09), and eyes with VA ≥0 .7 had the smallest (SDME logMAR 0.04). The variability may be partly explained by the line size progression in lower VAs, partly by the difference in the remea surement of the refractive error. The difference in the average VA between exami nations 1 and 2 (logMAR 0.15 versus 0.12) was considered to be of some interest because it indicates that some learning effect is possible. Conclusion: Visual a cuity results in clinical settings have a certain degree of inherent variability . In this series variability ranged from SDME logMAR 0.04 (eyes with good vision ) to logMAR 0.09 (in the lower vision group) in the Snellen VA range of 0.3-1.3 . Changes should be judged with caution, especially in cases of decreased VA. Methods: To estimate the random measurement error in visual acuity (VA) determ ination in the clinical environment in cataractous, pseudophakic and healthy eye s. Methods: The VAs of patients referred for cataract surgery or consultation by ophthalmic professionals were re-examined and the VA results for distance usin g projector acuity charts were compared. Refractive errors were also remeasured. A total of 99 eyes (41 cataractous, 36 pseudophakic and 22 healthy eyes) were e xamined. The healthy comparison group consisted of hospital staff. Only one eye The mean time interval between the first and second examinations was 45 days. Results: The estimated standard deviation of measurement error (SDME) of repeated Eyes with the lowest VA (0.3-0.45) had the largest variability (SDME logMAR 0.09), and eyes with VA ≥0.7 had the smallest (SDME logMAR 0.04). The variability may be partly explained by the line size progression in lower VAs, partly by the difference in the remea surement of the refractive error. The difference in the average VA between exami nations 1 and 2 (log MAR 0.15 versus 0.12) was considered to be of this interest because it indicates that some learning effect is possible. Conclusion: Visual a cuity results in clinical settings have a certain degree of inherent variability. In this series variability ranged from SDME logMAR 0.04 (eyes with good vision) to log MAR 0.09 (in the lower vision group) in the Snellen VA range of 0.3-1.3. Changes should be judged with caution, especially in cases of decreased VA.