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目的:研究β-内啡肽指标在脑内的表达变化,观察银杏叶制剂对致痛大鼠脑内β-内啡肽的影响。方法:取SD雌性大鼠50只,随机分为5组,模型组、药物对照组、给药组(高、中、低)三个剂量组。药物对照组给予生理盐水10天,给药组给与高中低三个剂量组的银杏叶的混悬液,每天给药两次,给药10天后,在大鼠的右脚掌给与5%的福尔马林150μl刺激,1小时后将大鼠处死,取海马和下丘脑做连续切片后,采用HE染色,在光镜下进行定位,用β-内啡肽做免疫组化染色,对照图谱在显微镜下观察β-内啡肽在海马区及下丘脑的阳性细胞数。结果:高、中剂量给药组中下丘脑室旁核、室周核、弓状核内β-内啡肽表达增加(P<0.05)。海马的CA1CA2、CA3区β-内啡肽的表达增加主要在高、中剂量组(P<0.05),低剂量组无差异。结论:大鼠给予银杏叶片制剂后,炎性致痛大鼠海马区及下丘脑区的β-内啡肽表达发生了变化,揭示银杏叶片在疼痛大鼠的中枢神经系统对β-内啡肽有一定的影响作用;提示银杏叶镇痛的有一定的中枢机制。
Objective: To study the expression changes of β-endorphin in the brain and observe the effects of Ginkgo biloba extract on brain β-endorphin in rats. Methods: Fifty SD female rats were randomly divided into 5 groups: model group, drug control group, and drug administration group (high, middle and low). The drug control group was given physiological saline for 10 days. The administration group was given a suspension of Ginkgo biloba leaves in the high, medium, and low dose groups, twice daily, and after 10 days of administration, the right foot of the rat was given 5% of the dose. Formalin 150μl stimulation, 1 hour after the rats were sacrificed, the hippocampus and hypothalamus were serially sectioned, HE staining, positioning under light microscopy, immunohistochemical staining with β-endorphin, control map The number of positive cells of β-endorphin in the hippocampus and hypothalamus was observed under a microscope. Results: The expression of β-endorphin in the hypothalamic paraventricular nucleus, periventricular nucleus and arcuate nucleus was increased in the high and middle dose groups (P<0.05). The expression of β-endorphin in the CA1CA2 and CA3 regions of the hippocampus increased mainly in the high- and middle-dose groups (P<0.05), but there was no difference in the low-dose group. Conclusion: The expression of β-endorphin in the hippocampus and hypothalamus of rats with inflammatory pain was changed after administration of Ginkgo biloba leaves in rats. It was revealed that ginkgo leaf was found to be β-endorphin in the central nervous system of pain rats. It has a certain influence; it suggests that there is a certain central mechanism for analgesia of Ginkgo biloba.