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目的:研究亚甲基四氢叶酸还原酶(MTHFR)基因多态性与冠心病的关系。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析检测224例宁夏回族人群(冠心病组107例,对照组117例)MTH-FR基因型及等位基因频率。结果:回族冠心病组和对照组MTHFRC677TCC,CT,TT3种基因型分布(χ2=5.523,P=0.063)和等位基因频率分布(χ2=0.000,P=0.984)差异无统计学意义(P>0.05)。结论:MTHFR基因多态性与冠心病无相关。
Objective: To study the relationship between methylenetetrahydrofolate reductase (MTHFR) gene polymorphism and coronary heart disease. Methods: The genotype and allele frequencies of MTH-FR in 224 Ningxia Hui people (107 in CHD group and 117 in control group) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: There were no significant differences in genotype distributions of MTHFRC677TCC, CT and TT between CHD patients and controls (χ2 = 5.523, P = 0.063) and allele frequencies (χ2 = 0.000, P = 0.984) 0.05). Conclusion: MTHFR gene polymorphism has no correlation with coronary heart disease.