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20 0 2年 6月中旬 ,在四川省攀枝花市和泸州市 ,从 3例急性弛缓性麻痹 (AFP)病例及其 3名密切接触者中 ,共分离到 6株脊髓灰质炎 (脊灰 )病毒。这 6人中有 5人是口服脊灰疫苗“零”剂次免疫 ,1名免疫史不详。用逆转录 聚合酶链反应 (RT PCR)分析病毒的VP1和 3D基因编码区发现 ,所有病毒均为疫苗重组脊灰病毒 (VRPV) ,其中 2株为Ⅱ型和Ⅰ型的重组 ,其余为Ⅱ型和Ⅲ型的重组。经序列分析发现 ,从AFP病例中分离到的毒株 ,在VP1编码区与标准参考株SabinⅡ型有 3个同样的核苷酸发生了变异 ,而从密切接触者中分离到的毒株有 4个核苷酸发生了变异。 6株病毒来源相同 ,并已在免疫覆盖率低的地方引起儿童患病及播散。
In mid-June 2002, six poliovirus (polio) viruses were isolated from three acute flaccid paralysis (AFP) cases and three close contacts in Panzhihua and Luzhou, Sichuan Province. . Five of the six people were orally immunized with “zero” of oral polio vaccine and one was unknown. The VP1 and 3D gene coding regions of the virus were analyzed by reverse transcriptase-polymerase chain reaction (RT PCR). All of the viruses were vaccine-recombinant poliovirus (VRPV), of which two were type II and type I and the rest were type II Type and type III recombination. Sequence analysis showed that the strains isolated from AFP mutated 3 identical nucleotides in the VP1 coding region and the standard reference strain Sabin II, whereas the strains isolated from close contacts had 4 A nucleotide mutation occurred. The 6 strains of viruses have the same origin and have caused children’s illnesses and spread in places with low immunocompetent coverage.