论文部分内容阅读
目的传统的影像学检查及肿瘤标志物检测对结直肠癌微转移的诊断价值有限。本研究旨在探讨ERCC1 mRNA在结直肠癌患者外周血中的表达情况及其临床意义。方法用实时定量荧光逆转录聚合酶链反应(RT-PCR)方法,对53例结直肠癌患者和21名健康人的外周血标本进行ERCC1 mRNA检测,分析其表达与肿瘤临床病理特征的关系并应用接受者操作特征曲线(ROC曲线)分析ERCC1 mRNA检测对结直肠癌的诊断价值。结果结直肠癌组外周血ERCC1 mRNA表达水平显著高于正常对照组(P<0.05),其表达与肿瘤淋巴结转移、血行转移和TNM分期相关(P<0.05)。ERCC1 mRNA诊断结直肠癌的ROC曲线下面积0.89,诊断界值为Ct≤32。结论实时定量荧光RT-PCR检测ERCC1 mRNA可以更好地评估结直肠癌血道微转移,有利于判断预后。
Purpose Traditional imaging examination and detection of tumor markers for the diagnosis of micrometastases in colorectal cancer are of limited value. This study aimed to investigate the expression of ERCC1 mRNA in peripheral blood of patients with colorectal cancer and its clinical significance. Methods Real-time quantitative fluorescent reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect the expression of ERCC1 mRNA in 53 colorectal cancer patients and 21 healthy volunteers. The relationship between ERCC1 mRNA expression and clinicopathological features was analyzed. The receiver operating characteristic curve (ROC curve) was used to analyze the diagnostic value of ERCC1 mRNA in colorectal cancer. Results The expression of ERCC1 mRNA in colorectal cancer group was significantly higher than that in normal control group (P <0.05). The expression of ERCC1 mRNA was correlated with lymph node metastasis, hematogenous metastasis and TNM stage (P <0.05). The area under the ROC curve of ERCC1 mRNA in diagnosing colorectal cancer was 0.89, and the diagnostic cutoff value was Ct≤32. Conclusion Real-time quantitative fluorescent RT-PCR detection of ERCC1 mRNA can better assess micrometastasis of colorectal cancer, which is good for predicting prognosis.