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目的研究芦丁对心肌纤维化大鼠ColⅠ、ColⅢ、TGF-β1的调控作用及其机制的变化。方法出生1 d Wistar大鼠6只,将大鼠分别贴上序号1~6,1、2号大鼠为模型组(用含有血管紧张素Ⅱ(AngⅡ)(9~5 mol/L)培养液来培养细胞);3、4号大鼠为芦丁组(在模型组的培养条件下加入芦丁,配成含量是5 mg/L培养细胞液);5、6号大鼠为正常对照组(用含有16%胎牛血清DMEM的培养液培养)。用ELISA法检测培养上清中ColⅠ和ColⅢ的含量和Western blotting法检测转化生长因子-β1(TGF-β1)蛋白表达。结果 ELISA检测显示,AngⅡ作用心肌纤维细胞24 h后,模型组细胞培养的上清液内ColⅢ和ColⅠ的含量较对照组明显上升(P<0.01);经芦丁治疗后,ColⅢ和ColⅠ的含量较模型组均明显下降(P<0.01)。Western blotting结果显示,使用AngⅡ激发心肌纤维细胞24 h以后,模型组的TGF-β1蛋白表达较对照组显著上升(P<0.01),经芦丁培养后,TGF-β1蛋白较模型组显著下降(P<0.01)。结论芦丁可以调控ColⅠ、ColⅢ、TGF-β1的蛋白表达,从而控制大鼠心肌纤维化。
Objective To study the regulatory effect of rutin on ColⅠ, Col Ⅲ and TGF-β1 in rats with myocardial fibrosis and its mechanism. Methods Six Wistar rats were born on the 1st day. The rats were respectively assigned with the numbers 1 ~ 6, 1 and 2 as model group (treated with angiotensin Ⅱ (9 ~ 5 mol / L) 3 and 4 rats were rutin group (rutin was added under the culture conditions of the model group, and the content of the rutin was 5 mg / L cultured cell liquid); rats 5 and 6 were normal control group (Cultured in a medium containing 16% fetal bovine serum DMEM). The contents of ColⅠ and Col Ⅲ in culture supernatant were detected by ELISA and the protein expression of transforming growth factor-β1 (TGF-β1) was detected by Western blotting. Results The results of ELISA showed that the contents of Col Ⅲ and ColⅠ in the cultured supernatant of model group increased significantly (P <0.01) after treated with AngⅡ for 24 h. After treatment with rutin, the content of Col Ⅲ and ColⅠ Compared with the model group were significantly decreased (P <0.01). The results of Western blotting showed that TGF-β1 protein expression in model group increased significantly (P <0.01) 24 hours after stimulated with AngⅡ, and the expression of TGF-β1 protein decreased significantly after rutin culture P <0.01). Conclusion Rutin can regulate the expression of ColⅠ, ColⅢ and TGF-β1 and thus control the myocardial fibrosis in rats.