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为探讨复方861抗肝纤维化的药理作用,研究其对活化的肝星状细胞系(HSC-T6)超微结构及细胞骨架蛋白的影响。一、材料与方法1.HSC-T6的复苏、培养及分组:HSC-T6(美国旧金山总医院肝病研究中心Friedman教授惠赠),为SV40转染SD大鼠HSCs。细胞于37℃快速复苏,以含10%小牛血清的DMEM (美国Gibco BRC公司)制备为1×105个细胞/ml悬液,接种于35 mm细胞培养皿(皿内先放置20 mm×20 mm的盖玻片) 中,培养基总体积为2 ml。在95%O2+5%CO2,37℃条件下,HSCs于CO2培养箱内24 h,细胞完全贴壁后进行以下分
To investigate the pharmacological effects of Compound 861 on hepatic fibrosis, the effects of compound 861 on the ultrastructure and cytoskeletal proteins of activated hepatic stellate cell line (HSC-T6) were studied. I. Materials and Methods 1. HSC-T6 resuscitation, culture and grouping: HSC-T6 (gift given by Prof. Friedman, Center for Liver Research, San Francisco General Hospital, USA). SD rat HSCs were transfected with SV40. Cells were rapidly resuscitated at 37°C and prepared as a 1×105 cells/ml suspension in DMEM (Gibco BRC, USA) containing 10% fetal bovine serum. The cells were inoculated into 35 mm cell culture dishes (20 mm×20 was placed in the dish first). In a cover glass of mm), the total volume of the culture medium is 2 ml. Under the conditions of 95% O2+5%CO2 and 37°C, HSCs were incubated in a CO2 incubator for 24 h. After the cells were completely adherent, the following points were scored.