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非生物逆境,如低温、高温、干旱通常会严重影响到农作物的生长及产量,其中高温胁迫则是造成植物伤害的主要原因之一。为深入了解水稻高温胁迫反应的分子机理,发现新的耐高温相关功能基因,为水稻生物工程育种提供候选材料,采用Affymetrix水稻表达芯片分析了超级稻两优培九母本培矮64S(Oryzasativa L.)在高温逆境胁迫下,孕穗期、抽穗开花期的叶片全基因组表达谱,得到大量高温诱导表达基因。应用实时定量PCR方法对其中一部分基因的表达水平进一步分析,所得结果与基因芯片结果基本吻合,证明芯片分析数据是可靠的,为下一步耐高温相关基因的克隆、功能分析等研究提供了基础。
Abiotic stresses, such as low temperature, high temperature, and drought usually have a serious impact on crop growth and yield. High temperature stress is one of the major causes of plant damage. In order to further understand the molecular mechanism of high temperature stress response in rice and to find out new functional genes related to high temperature, we provide candidate materials for rice bioengineering. Affymetrix rice expression chips were used to analyze the effects of Oryzasativa L. Under high temperature stress, the whole genome expression profiles of leaves at booting, flowering and flowering stages were obtained, and a large amount of high temperature induced expression genes were obtained. The real-time quantitative PCR method was used to analyze the expression level of some of these genes. The results were in good agreement with the results of microarray analysis, which proved that the data of chip analysis was reliable and provided the basis for the cloning and function analysis of the genes related to high temperature in the next step.