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目的:研究重症肌无力(MG)患者CD40配体(CD40L)的表达,探讨CD40L在MG中的作用。方法:25例MG患者,分为急性期组13例,非急性期组12例,设正常对照组15例。分离血中单个核细胞,分别培养并用不同刺激剂(PHA、PMA和A23187)刺激。培养前后用流式细胞仪检测CD40L阳性细胞率。结果:①MG急性期组CD40L阳性细胞率比正常对照组及非急性期组显著增高(均为P<0.01);②经PHA或PMA和A23187刺激后,非急性期组CD40L阳性细胞率也比正常对照组显著增高(P<0.01)。结论:CD40L的高表达与MG发生和加重有关。
Objective: To investigate the expression of CD40 ligand (CD40L) in patients with myasthenia gravis (MG) and to explore the role of CD40L in MG. Methods: 25 patients with MG were divided into acute phase group (n = 13), non-acute phase group (n = 12) and normal control group (n = 15). Blood mononuclear cells were isolated and cultured separately and stimulated with different stimulants (PHA, PMA and A23187). Before and after culturing, the rate of CD40L positive cells was detected by flow cytometry. Results: ①The positive rate of CD40L positive cells in acute phase of MG was significantly higher than that of normal control and non-acute phase (all P <0.01); ② After stimulation with PHA or PMA and A23187, the positive rate of CD40L in non- The control group was significantly higher (P <0.01). Conclusion: The high expression of CD40L is associated with the occurrence and aggravation of MG.