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本文报导了胎儿血小板促凝血素(TXA_2)的生成以及四种常用的前列腺素抑制剂(PGSI_s)对TXA_2的生成和对人脐动脉合成前列腺环素(PGⅠ_2)的作用。从13名产自健康母亲(孕期未服过PGSI_s)的婴儿取2 ml血置于含0.2ml3.8%枸橼酸钠的真空试管中。对照管先加0.1mlS(?)rensen氏缓冲液(pH7.4),实验管则先加不同剂量的PGSI_s:阿斯匹林、消炎痛、Naproxen和Diclofenac于0.1mlS(?)rensen氏缓冲液中,使其最终浓度为10~(-8)～10~(-3)。用力混合,在37℃保温15分钟,用0.1ml200mM/lCaCl~2(在154mM/l盐水中)中和枸橼酸钠,于
This article reports the generation of TXA_2 and the effects of four commonly used prostaglandin inhibitors (PGSI_s) on TXA_2 and prostacyclin (PGⅠ_2) production in human umbilical arteries. Two ml of blood from 13 infants born to healthy mothers who had not taken PGSI_s during pregnancy was placed in a vacuum tube containing 0.2 ml of 3.8% sodium citrate. The control tube was initially filled with 0.1 ml of rensen’s buffer (pH 7.4), and the experimental tube was first treated with different doses of PGSI_s: aspirin, indomethacin, Naproxen and Diclofenac in 0.1 ml of rensen’s buffer In the final concentration of 10 ~ (-8) ~ 10 ~ (-3). Mix vigorously, incubate at 37 ° C for 15 minutes, neutralize sodium citrate with 0.1 ml of 200 mM / l CaCl 2 (in 154 mM / l saline)