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背景:大量研究表明神经因素可调节骨代谢,迄今为止已发现5种神经肽参与骨代谢过程。目的:观察正常人成骨细胞表面不同神经肽受体的表达。方法:分别以降钙素基因相关肽、酪氨酸羟化酶、P物质、神经肽Y的单克隆抗体进行免疫组化染色,观察各种肽类受体在成骨细胞表面的表达情况。另外,利用计算机图像分析系统对染色灰度进行半定量分析。结果与结论:正常人成骨细胞表面有神经肽Y、P物质、酪氨酸羟化酶、降钙素基因相关肽受体表达,这些因子可以通过与相应的受体结合影响细胞生物学特性。不同因子免疫组化染色的灰度值由小到大依次为神经肽Y、降钙素基因相关肽、酪氨酸羟化酶、P物质,神经肽是成骨细胞活性的重要调节因子。
Background: Numerous studies have shown that neurological factors regulate bone metabolism. To date, five neuropeptides have been found to be involved in bone metabolism. Objective: To observe the expression of different neuropeptide receptors on the surface of normal human osteoblasts. Methods: The expression of calcitonin gene - related peptide, tyrosine hydroxylase, substance P and neuropeptide Y were detected by immunohistochemistry and the expression of various peptide receptors on the surface of osteoblasts was observed. In addition, the computer image analysis system for semi-quantitative analysis of staining gray. RESULTS AND CONCLUSION: The expression of neuropeptide Y, substance P, tyrosine hydroxylase and calcitonin gene-related peptide receptors on the surface of normal human osteoblasts can affect the biological characteristics of the cells through their binding to the corresponding receptors . The gray value of immunohistochemical staining of different factors followed by neuropeptide Y, calcitonin gene related peptide, tyrosine hydroxylase, substance P and neuropeptide were important regulators of osteoblast activity.