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为探讨双孢蘑菇子实体发育后期的蛋白质表达变化,对双孢蘑菇As2796子实体采收期、成熟期和开伞期的蛋白质组进行了双向电泳(2-DE)分析,发现了16个表达差异明显的蛋白质。通过质谱分析(MALDI-TOF/TOF MS)和数据库检索,有14个差异蛋白质获得鉴定。其中磷酸烯醇式丙酮酸水合酶与能量代谢相关,T-蛋白复合体1、蛋白酶体、5-甲基四氢三谷氨酸-同型半胱氨酸甲基转移酶、1-吡咯琳-5-羧酸脱氢酶、精氨酸酶与氨基酸或蛋白质代谢直接相关,而GTP结合蛋白则参与细胞的多种生命活动,在细胞的生长发育过程中起着重要的作用。另外7个为功能未知的蛋白质。
In order to investigate the changes of protein expression in the late fruiting bodies of Agaricus bisporus, two-dimensional electrophoresis (2-DE) analysis was performed on the proteome of harvested, mature and unfolding phases of As2796 fruiting bodies of Agaricus bisporus, and 16 proteins were found Significant differences in the protein. Fourteen differential proteins were identified by mass spectrometry (MALDI-TOF / TOF MS) and database searches. Among them, phosphoenolpyruvate hydratase is related to energy metabolism, and T-protein complex 1, proteasome, 5-methyltetrahydrotalcidyl-homocysteine methyltransferase, 1-pyrroline- - Carboxylate dehydrogenase, arginase and amino acids or protein metabolism is directly related to, and GTP binding protein is involved in a variety of cell life activities, plays an important role in cell growth and development. The other seven are unknown proteins.