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本文采用~3H-TdR参入法和羟基磷灰石层析法,比较了~(60)Coγ射线照射后PHA、ConA、PWM激活的人血淋巴细胞的转化、DNA单链断裂及其修复,结果表明:受照后淋巴细胞转化受抑,在0—8Gy剂量范围内,剂量效应呈双相线性关系,其中PWM激活的细胞对射线的敏感性最低。三种细胞受照后DNA发生单链断裂,在0—30Gy范围内,与剂量呈线性相关,三者之间无显著差异。受15Gy照射后,细胞在37℃条件下能重接DNA断链,但重接不完全,重接后如经较长时间保温仍会发生再断裂,PWM激活的细胞重接修复率最高。淋巴细胞转化对辐射的敏感性可能与DNA断链的重接修复能力有关。
In this study, ~3H-TdR incorporation and hydroxyapatite chromatography were used to compare the conversion of PHA, ConA and PWM-activated human blood lymphocytes, DNA single-strand breaks and their repair after ~(60)Co gamma-ray irradiation. The results showed that after irradiation, lymphocyte transformation was inhibited. In the 0-8 Gy dose range, the dose effect showed a biphasic linear relationship, in which the PWM-activated cells had the lowest sensitivity to radiation. Single-stranded DNA breaks in the three cells after exposure to radiation. Within the range of 0-30 Gy, there is a linear correlation with the dose, and there is no significant difference between the three. After irradiation with 15Gy, the cells can reconnect DNA strands at 37°C, but the rejoins are incomplete. If the cells are re-applied for a long time, re-disruption will occur, and the reactivation rate of the cells activated by PWM is the highest. The sensitivity of lymphocyte transformation to radiation may be related to the repetitive repair ability of DNA strand breaks.