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以荧光光谱为手段,研究了药物利复星(Levofloxacin)与牛血清白蛋白(BSA)的作用和影响,研究了它的荧光淬灭现象。在向该溶液滴加利复星时,观察不到激发峰的明显移动,而发射峰出现了新颖的现象, 原有的353．1 nm的发射峰强度明显的减弱;新出现了峰位位于450．4 nm的新的荧光发射峰。利复星-BSA 体系的猝灭过程不是因为分子扩散和碰撞所引起的动态猝灭,而是分子之间结合形成了化合物所引起的静态猝灭。利复星的离解常数为Kd=3．39×10-5mol／L。利复星的能量转移效率为50％时给体和受体之间距离的 R0=1．81×10-7cm,利复星和牛血清白蛋白的能量转移效率为E=0．351,根据这些计算结果可以知道利复星和牛血清白蛋白的色氨酸残基的结合位置为r=2．01×10-7cm。
Fluorescence spectroscopy was used to study the effect and influence of Levofloxacin and bovine serum albumin (BSA) on fluorescence quenching. No significant shift of the excitation peak was observed during the dropping of the diphosphate into the solution, and the emission peak showed a novel phenomenon. The original emission peak intensity at 353.1 nm was significantly weakened. A new peak appeared at New fluorescence emission peak at 450.4 nm. The quenching process of the Fuso-BSA system is not due to the dynamic quenching caused by the molecular diffusion and collision but rather the quasi-static quenching caused by the compounds. Lee Fuli’s dissociation constant Kd = 3.39 × 10-5mol / L. Rf = 1.81 × 10-7 cm for the distance between the body and the receptor when the energy conversion efficiency of rifampicin was 50%, and E = 0.351 for the energy transfer efficiency of rifampicin and bovine serum albumin. According to these The calculation results show that the binding sites of the tryptophan residues of rifosun and bovine serum albumin are r = 2.01 × 10-7 cm.