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目的 观察高葡萄糖环境中 ,蛋白激酶C(PKC)抑制剂灯盏花素对肾小球系膜细胞 (GMC)c fos、c jun蛋白表达和Ⅳ型胶原 (C Ⅳ )合成的影响 ,探索糖尿病肾病防治的新途径。方法 原代培养大鼠GMC ,分别置于正常葡萄糖 (对照组 )、高葡萄糖 (高糖组 )和高葡萄糖加灯盏花素 (高糖加灯盏花素组 )环境中 ,观察干预 2 4h、4 8h和 1wk后GMCc fos、c jun蛋白表达、C Ⅳ合成和PKC活性的变化。结果 与对照组比较 ,高糖组干预 2 4h后c fos、c jun蛋白表达同时明显增高 ,4 8h后c fos开始下降 ,而c jun 1wk后仍保持高水平 ,高糖组C Ⅳ合成 1wk后增加 ,各观察时点PKC活性均较对照组明显增高 ;而高糖加灯盏花素组各时点c fos、c jun蛋白表达、C Ⅳ合成和PKC活性均低于高糖组。结论 高葡萄糖可促使GMC中c fos、c jun蛋白表达和C Ⅳ合成增加 ,此可能为PKC活化所介导 ,灯盏花素可通过抑制PKC活化而有效阻止高葡萄糖引起的上述变化。
Objective To investigate the effects of breviscapine, a protein kinase C (PKC) inhibitor, on the expression of c fos and c jun and the synthesis of collagen Ⅳ in glomerular mesangial cells (GMC) in a high glucose environment. To explore the effects of breviscapine on diabetic nephropathy A new way of prevention and cure. Methods Primary cultured rat GMCs were exposed to normal glucose (control group), high glucose (high glucose group), high glucose plus breviscapine (high glucose plus breviscapine) The expression of fos and c jun, the synthesis of C Ⅳ and the activity of PKC in GMCc after 8h and 1wk. Results Compared with the control group, the expression of c fos and c jun protein in the high glucose group increased significantly after 24 h, and began to decrease after 48 h and remained high after c jun 1 wk, While the activity of PKC at each observation time was significantly higher than that of the control group. However, the expression of c fos and c jun protein, the synthesis of C Ⅳ and the activity of PKC in high glucose and breviscapine group were lower than those in high glucose group at each time point. Conclusion High glucose can promote the expression of c fos, c jun and increase the synthesis of C Ⅳ in GMC, which may be mediated by PKC activation. Breviscapine can effectively prevent the above changes caused by high glucose by inhibiting PKC activation.