干旱和NaCl胁迫下木薯GB含量及BADH基因的表达

来源 :农业生物技术学报 | 被引量 : 0次 | 上传用户:jimmil
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
甜菜碱醛脱氢酶(betaine aldehyde dehydrogenase,BADH)是甜菜碱(glycine betaine,GB)合成过程中的关键酶。为检测BADH基因在木薯(Manihot esculenta)不同组织中的表达差异,探究干旱与NaCl胁迫下其表达谱变化规律,本研究利用生物信息学方法分析和鉴定木薯BADH基因,定位MeBADHs在染色体上的位置以及确定5’端上游序列顺式作用元件;采用qRT-PCR测定BADH基因在木薯品种SC5根、块根、茎和叶中的表达差异,同时检测在PEG-6000模拟脱水胁迫、NaCl胁迫及自然干旱胁迫下MeBADHs基因的转录水平变化,采用相关试剂盒测定木薯中GB含量的变化。结果显示,MeBADHs两个基因在各组织之中均有表达,在块根中表达量显著高于根(P<0.05)。PEG-6000模拟干旱条件下,与0 h相比,1~2 h时MeBADH1和MeBADH2在叶、茎、根以及块根中的表达量变化不大;NaCl处理条件下,叶中MeBADH1在8 h表达量极显著高于0 h(P<0.01),而MeBADH2则在不同处理时间下的表达量水平相差不大,在茎和根中,MeBADHs在处理发生1 h时表达量水平极显著高于0 h(P<0.01);自然缺水干旱条件下,茎和根中MeBADH1和MeBADH2分别在6和18 d时的表达量极显著高于0 d(P<0.01)。PEG-6000处理下,GB含量出现了波动并呈现略增趋势;NaCl处理条件下GB含量在前期表达较稳定,8 h时剧烈响应,即出现下调趋势,24 h时GB含量显著低于0 h(P<0.05)。在自然干旱胁迫下,GB含量逐渐呈现上调趋势,并在9和15 d达到高峰,显著高于0 d(P<0.05)。研究结果为进一步研究木薯分子抗旱机制及新品种的选育提供了理论依据。 Betaine aldehyde dehydrogenase (BADH) is a key enzyme in the synthesis of glycine betaine (GB). In order to detect the difference of BADH gene expression in different tissues of Cassava (Manihot esculenta), we investigated the variation of BADH gene expression profile under drought and NaCl stress. In this study, bioinformatics methods were used to analyze and identify the BADH gene in Cassava and to locate the position of MeADHs on the chromosome And determine the cis-acting element upstream of the 5 ’end. The expression differences of BADH gene in root, shoot, stem and leaf of cassava variety SC5 were detected by qRT-PCR. Simultaneously, PEG-6000 simulated dehydration stress, NaCl stress and natural drought Under stress, the transcription level of MeBADHs gene was changed. The relative kit was used to determine the content of GB in cassava. The results showed that two genes of MeBADHs were expressed in all tissues, and the expression level of MeBADHs in root tuber was significantly higher than that in roots (P <0.05). Compared with 0 h, the expression levels of MeBADH1 and MeBADH2 in leaves, stems, roots and tubers did not change much at 1 ~ 2 h in PEG-6000 simulated drought conditions. MeBADH1 was expressed at 8 h after NaCl treatment The amount of MeBADH2 expression in the stems and roots at 1 h after treatment was significantly higher than that at 0 h (P <0.01) h (P <0.01). Under drought and drought conditions, the expression levels of MeBADH1 and MeBADH2 in stem and root at 6 and 18 d were significantly higher than those at 0 d (P <0.01). Under the treatment of PEG-6000, the content of GB fluctuated and showed a slight increase trend. Under NaCl treatment, the content of GB was stable in the early stage and responded strongly at 8 h, indicating the downward trend. The content of GB at 24 h was significantly lower than 0 h (P <0.05). Under natural drought stress, the content of GB gradually increased and reached the peak at 9 and 15 d, significantly higher than that at 0 d (P <0.05). The results provide a theoretical basis for further research on cassava molecular mechanism of drought resistance and selection of new varieties.
其他文献
长期以来,我国的“三农”问题倍受关注。解决“三农”问题的关键就是农村土地制度问题。目前,我国农业生产正处于专业化、规模化和市场化不断深入发展的阶段,农民的生活方式和农
本文通过对荣华二采区10
期刊
20世纪90年代以来,区域经济一体化进程加快,国际垂直专业化深入发展并引起了生产链条的国际分布。积极参与国际分工,是发展中国家促进经济发展、开辟其产业培育路径及推动其产业
本文运用统计学、管理学、法经济学和法社会学法等学科知识,对近年来东莞市外资企业主恶意欠债弃厂逃匿问题进行了系统地分析和实证性地研究。外资企业欠债逃匿是指外资企业在
摘 要:为解决钱家营矿选煤厂浮选精煤产率低、灰分高的问题,对其工艺系统和设备进行改造。通过煤泥小筛分、小浮沉和可浮性分析,确定灵活的工艺流程,且在改造中应用较先进的多次选和分级选浮选工艺系统,同时采用性能先进的大型浮选、脱水设备。工艺改造后,该厂精煤产率和尾煤灰分得到明显提高提高。  关键词:浮选工艺 多次选 分级选 工艺改造  中图分类号:TD946.2 文献标识码:A 文章编号:1007-39
大疆无疑是无人机行业的领头羊,旗下的无人机飞行器大都以高品质著称.近日,大疆在2018年年底推出的旗下电影级别的航拍平台STORM,现在终于在大疆的官网上现身.rn据了解,在这
期刊
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生、测量监控等方面人手,介绍了S226海滨大桥
新航线的开辟以及新大陆的发现促进了全球贸易的迅速扩张。伴随海上贸易的扩张,16-19世纪也是全球海盗的黄金时代。但是海盗这个名词并不能掩盖东西方海盗活动性质的差异以及
●办公互环境智能控制系统    1. 会议室全部灯光实现智能化  1)主持人可以用手中的遥控器任意控制任何一路灯光  2)场景灯光:按动遥控器上的场景键,顷刻实现如下灯光模式  ◆电视会议场景灯光:灯光路数开启较多,光源相对充足,营造热烈的会议气氛。  ◆投影播放场景灯光:主光源开启较少或者不开启,辅助灯带或局部筒灯作为主要光源。  ◆会议休息场景灯光:主光源开启较少,光源照度介于上述两种模式之间