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【目的】调查白芨内生细菌种类及多样性,为更好地保护和开发利用白芨资源提供科学依据。【方法】应用Illumina Mi Seq高通量测序技术测定白芨内生细菌的16S r DNA-V4变异区序列,应用Qiime和Mothur等软件整理和统计样品序列数目和操作分类单元(OTUs)数量,分析物种丰度及分布的差异。【结果】获得用于分析的有效序列和OTU数为49550/48;稀释曲线表明测序深度充分,OTU的数量接近于饱和。白芨内生细菌主要分布于鞘脂单胞菌属(Sphingomonas,32.26%)、地杆菌属(Pedobacter,16.13%)、盐单胞菌属(Halomonas,16.13%)、土壤杆菌属(Agrobacterium,12.90%)、Kaistobacter属(6.45%)、希瓦氏菌属(Shewanella,6.45%)、假单胞菌属(Pseudomonas,6.45%)和短波单胞菌属(Brevundimonas,3.23%)8个属。【结论】鞘脂单胞菌属、地杆菌属、盐单胞菌属和土壤杆菌属是白芨内生细菌的优势种群。Illumina Mi Seq高通量测序技术能准确反映植物内生微生物的种类组成和真实比例,在植物内生微生物研究中具有明显的优势和可行性。
【Objective】 The objective of this study was to investigate the species and diversity of endophytic bacteria in white catfish and provide a scientific basis for better protection and exploitation of catfish. 【Method】 The sequence of 16S r DNA-V4 variation in endophytic bacteria was determined by using Illumina Mi Seq high-throughput sequencing. The number of sequencing and number of OTUs were analyzed and quantified using Qiime and Mothur softwares. Differences in abundance and distribution. [Results] The number of effective sequences and OTUs obtained for analysis was 49550/48; the dilution curve showed that the sequencing depth was sufficient and the number of OTU was close to saturation. The endophytic bacteria were mainly distributed in Sphingomonas (32.26%), Pedobacter (16.13%), Halomonas (16.13%), Agrobacterium (12.90% , 8 genera of Kaistobacter (6.45%), Shewanella (6.45%), Pseudomonas (6.45%) and Brevundimonas (3.23%). 【Conclusion】 Sphingomonas, Geobacillus, Halomonas and Agrobacterium are the dominant populations of endophytic bacteria. Illumina Mi Seq high-throughput sequencing technology can accurately reflect the species composition and the true proportion of plant endogenous microorganisms, plant endophytic microorganisms in the study has obvious advantages and feasibility.