论文部分内容阅读
目的:检测广州地区沙眼衣原体临床分离菌株对阿奇霉素敏感性及常见耐药基因。方法:收集Mc Coy细胞培养阳性的111株沙眼衣原体临床菌株,PCR法扩增阿奇霉素耐药相关23S rRNA基因、核糖体蛋白L4基因以及L22基因,进行电泳并测序;随机选取46株临床菌株进行阿奇霉素药敏试验。结果:111株沙眼衣原体均未见常见耐药基因突变。46株沙眼衣原体对阿奇霉素最小抑菌浓度(MIC)为0.08~0.32μg/mL,其中MIC_(50)、MIC_(90)分别为0.08μg/mL、0.16μg/mL。结论:广州地区沙眼衣原体临床分离菌株对阿奇霉素敏感且无常见耐药基因突变。
Objective: To detect azithromycin susceptibility and common resistance gene of clinical isolates from Chlamydia trachomatis in Guangzhou. Methods: The clinical isolates of 111 C. trachomatis isolates positive for Mc Coy cell culture were collected. The 23S rRNA gene, ribosomal protein L4 gene and L22 gene were amplified by polymerase chain reaction (PCR) and sequenced. 46 clinical isolates were randomly selected for azithromycin drug allergy testing. Results: 111 strains of Chlamydia trachomatis were not common resistance gene mutations. The minimum inhibitory concentration (MIC) of 46 strains of Chlamydia trachomatis on azithromycin was 0.08 ~ 0.32μg / mL, the MIC_ (50) and MIC_ (90) were 0.08μg / mL and 0.16μg / mL respectively. Conclusion: The isolates of Chlamydia trachomatis isolates from Guangzhou are sensitive to azithromycin and there are no common mutations in the gene.