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目的研究成人急性淋巴细胞白血病(ALL)的免疫表型特征,并分析免疫表型与细胞形态学及细胞遗传学的关系。方法应用一组系列相关单克隆抗体和CD45／SSC设门的三色流式细胞术对50例初治成人ALL进行免疫表型研究。结果 50 例ALL中42例B系来源,5例T系ALL,3例T／B混合型ALL。27例伴有髓系抗原表达(54％),髓系抗原主要表达CD13、CD33。大部分病例(90％)幼稚细胞均出现CD45的缺失及弱表达。细胞学形态学分析发现所分析病例中FAB-L1占多数(67％)、L2 27 ％。2例L3其免疫表型为B-祖细胞及成熟B细胞表型。20例存在染色体异常,其中6例染色体数目异常,涉及的异常为 +8、-X、-7、-11、-4、、6和、13。染色体结构异常最多见为t(9;22),且均伴有髓系抗原的表达。结论 ALL免疫表型不是单独的诊断标准,其与细胞形态学及遗传学密切相关。
Objective To investigate the immunophenotypic characteristics of adult acute lymphoblastic leukemia (ALL) and to analyze the relationship between immunophenotype and cell morphology and cytogenetics. Methods A series of monoclonal antibodies and CD45 / SSC portal tri-color flow cytometry was used to study the immunophenotype of 50 newly diagnosed adult ALL patients. Results Forty-two cases of B-type origin in 50 cases of ALL, 5 cases of T-ALL and 3 cases of T / B mixed ALL. 27 cases were associated with myeloid antigen expression (54%), myeloid antigens mainly expressed CD13, CD33. The majority of naive cells (90%) showed deletion and weak expression of CD45. Cytological morphological analysis revealed a majority (67%) of FAB-L1 and 27% of L2 in the analyzed cases. Two cases of L3 immunophenotype for the B-progenitor cells and mature B cell phenotype. Chromosome abnormalities were present in 20 cases, of which 6 were abnormal in chromosome number and involved abnormalities of +8, -X, -7, -11, -4, 6, and 13. Chromosome structural abnormalities most often seen as t (9; 22), and are associated with myeloid antigen expression. Conclusion ALL immunophenotype is not a separate diagnostic criteria, which is closely related to cell morphology and genetics.