论文部分内容阅读
目的泄浊解毒、活血通络中药对糖尿病肾病大鼠肾脏MMP-9/TIMP-1影响的实验研究。方法予高糖高脂饲料喂养,并一次性予大鼠腹腔注射链脲佐菌素(streptozotocin,STZ)(35 mg/kg),建立糖尿病模型。将成模大鼠随机分成中药组、厄贝沙坦组、中药+厄贝沙坦组、模型组,并设立正常对照组,治疗16周。用免疫组化法检测MMP-9、TIMP-1在肾脏的表达。结果泄浊解毒、活血通络中药可抑制糖尿病大鼠肾组织TIMP-1的表达、上调肾组织MMP-9的表达,促进细胞外基质的降解而拮抗糖尿病肾病肾小球硬化,减轻肾脏慢性损伤,延缓糖尿病肾病(diabetic nephropath)进一步发展。结论泄浊解毒、活血通络中药可能通过影响糖尿病大鼠肾脏MMP-9、TIMP-1的表达而起到保护糖尿病大鼠肾脏的作用。
Objective To study the effect of Xiezhuojiedu and Huoxuetongluo on the renal MMP-9 / TIMP-1 in rats with diabetic nephropathy. Methods High glucose and high fat diet was given to rats and the rats were injected intraperitoneally with streptozotocin (STZ) (35 mg / kg) to establish diabetic model. The model rats were randomly divided into traditional Chinese medicine group, irbesartan group, traditional Chinese medicine + irbesartan group and model group, and normal control group was established for 16 weeks. Immunohistochemistry was used to detect the expression of MMP-9 and TIMP-1 in the kidney. Results Xiezhuojiedu and Huoxuetongluo can inhibit the expression of TIMP-1 in renal tissue of diabetic rats, increase the expression of MMP-9 in renal tissue, promote the degradation of extracellular matrix, antagonize the glomerulosclerosis of diabetic nephropathy and reduce the chronic injury of the kidney , Delay the development of diabetic nephropath. Conclusions: Xiezhuojiedu and Huoxuetongluo Chinese herbs may protect the kidneys of diabetic rats by affecting the expressions of MMP-9 and TIMP-1 in the kidney of diabetic rats.