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冈 4 6B(G4 6B)是水稻生产应用中的一个农艺性状十分优良的保持系 ,其主要的缺陷是稻瘟病抗性较弱 ,通过对地谷 ,BL 1,Pi 4号等三个分别含抗病基因Pi d(t) 1 、Pi b、Pi ta2 的稻瘟病抗性材料与G4 6B聚合杂交 ,并利用抗病基因连锁的分子标记对杂交后代进行辅助选择 ,在聚合杂交的F2代及B1C1代群体中共获得了 15株含Pi d(t) 1 、Pi b、Pi ta2 等三个抗稻瘟病基因的材料 ,其可能的基因型分别为 :三基因杂合体Pi d(t) 1 pi d(t) 1 Pi bpi b Pi ta2 pi ta2 4株 ,双基因杂合体 10株 ,其中Pi d(t) 1 Pi d(t) 1 Pi bpi b Pi ta2 pi ta2 6株 ,Pi d(t) 1 pi d(t) 1 Pi bpi b Pi ta2 Pi ta2 3株 ,Pi d(t) 1 pi d(t) 1 Pi bPi b Pi ta2 pi ta2 1株 ,双基因纯合体Pi d(t) 1 Pi d(t) 1 Pi bpi b Pi ta2 Pi ta2仅 1株 ,这一研究结果为进一步改良G4 6B的稻瘟病抗性奠定了基础 ,同时这一研究结果表明利用分子标记可快速、有效地实现多个抗病基因的聚合 ,大大提高水稻抗病育种的效率
Gang 4 6B (G4 6B) is a very good maintainer line of agronomic traits in rice production. Its main defect is that the blast resistance is weak. The rice blast resistance materials Pi d (t) 1, Pi b and Pi ta2 were crossed with G4 6B and molecular markers linked with disease resistance genes were used to assist the selection of hybrid progenies. In the F2 generation and A total of 15 materials containing Pi d (t) 1, Pi b, Pi ta2 and other blast resistance genes were obtained from B1C1 generation. The possible genotypes were: Pi d (t) 1 pi There were 4 strains of d (t) 1 Pi bpi b Pi ta2 pi ta2 and 10 strains of double gene hybrid Pi d (t) 1 Pi d (t) 1 Pi bpi b Pi ta2 pi ta2 6 strains, Pi d (t) 1 pi d (t) 1 Pi bpi b Pi ta2 Pi ta2 3 strains Pi d (t) 1 pi d (t) 1 Pi bPi b Pi ta2 pi ta2 1 strain, Double gene homozygote Pi d (t) 1 Pi The results of this study laid the foundation for further improvement of the blast resistance of G4 6B. At the same time, the results of this study showed that the molecular marker can be used to achieve more and more rapid A resistance gene polymerization, Greatly improve the efficiency of rice breeding