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目的:建立10批不同产地火木层孔菌桑黄醇提物(EEPI)的HPLC指纹图谱,并对其质量进行评价。方法:采用SHISEIDO CAPCELL PAK C18色谱柱(250 mm×4.6 mm,5μm),以0.2%磷酸水溶液-甲醇进行梯度洗脱,流速1.0 mL/min,检测波长为395 nm。以Inoscavin A为参比峰建立EEPI特征吸收峰共有模式,并通过峰重叠率计算、相似度评价系统、聚类分析等对其相似度进行评价。结果:在选定的色谱条件下,得到10批不同产地火木层孔菌桑黄指纹图谱,标定了9个共有峰,并建立了火木层孔菌桑黄的指纹图谱共有模式,相似度在0.652~0.995之间,聚类分析结果将10批火木层孔菌桑黄分为3个大类。结论:此方法简单、准确、重复性好,峰分离度较好,为有效地评价火木层孔菌桑黄的综合质量提供了参考依据。
OBJECTIVE: To establish HPLC fingerprints of 10 batches of seedlings of Phellinus igniatus (EEPI) from different producing areas and to evaluate its quality. Methods: The gradient elution was performed on a SHISEIDO CAPCELL PAK C18 column (250 mm × 4.6 mm, 5 μm) with 0.2% phosphoric acid in methanol. The flow rate was 1.0 mL / min and the detection wavelength was 395 nm. The common mode of EEPI characteristic peak was established with Inoscavin A as reference peak, and its similarity was evaluated by peak overlap rate, similarity evaluation system and cluster analysis. Results: Under the selected chromatographic conditions, 10 fingerprints of Phellinus igniarius were obtained and 9 common peaks were calibrated. The common pattern of fingerprints of Phellinus igniatus was established, and the similarity Between 0.652 and 0.995, the results of cluster analysis divided 10 batches of Phellinus igniarius into three categories. Conclusion: This method is simple, accurate, reproducible and has good peak resolution. It provides a reference for effectively evaluating the comprehensive quality of Phellinus igniatus.