以ＲＵ４８６为内标，建立了血清样品中新型抗孕激素甾体药物利洛司酮（ｌｉｌｏｐｒｉｓｔｏｎｅ）的反相高效液相色谱法。色谱分析条件为μＢｏｎｄａｐａｋＣ１８柱１０μｍ，３００ｍｍ×３．９ｍｍＩＤ；流动相为甲醇－二氯甲烷－１０ｍｍｏｌ·Ｌ－１磷酸盐缓冲液（ｐＨ４．０）（６７：５：２８ｖ／ｖ）；紫外检测波长为３０２ｎｍ。利洛司酮的检测限为１ｎｇ（Ｓ／Ｎ≥４：１）。血清样品经二次液一液超声振荡萃取后，得到了较好的净化。血清样品中利洛司酮的方法回收率为１０３．３％，日内精密度及日间精密度平均ＲＳＤ为３．５１％及２．９２％。在浓度为１０～１０００ｎｇ·ｍｌ－１血清范围内呈线性关系。 Using RU486 as an internal standard, reverse-phase high performance liquid chromatography (HPLC) was developed for the determination of lilopristone, a new antiprogestational steroid drug in serum samples. The chromatographic conditions were μBondapak C18 column 10μm, 300mm × 3.9mmID; the mobile phase was methanol-dichloromethane-10mmol·L-1 phosphate buffer (pH 4.0) (67: 5: 28v / v) Is 302 nm. The limit of detection for lilopristin is 1 ng (S / N> 4: 1). Serum samples by the second liquid one ultrasonic oscillation extraction, get a better purification. The recovery of lilopristone in serum samples was 103.3%. The average intra-day and intraday precision RSDs were 3.51% and 2.92%, respectively. In the concentration range of 10 ~ 1000ng · ml-1 serum showed a linear relationship.