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目的 :探讨反义寡核苷酸 (AS -ODN)对缺氧 /再给氧 (H/R)时内皮细胞细胞间粘附分子 - 1(ICAM- 1)表达的影响。方法 :流式细胞仪测定肾小球血管内皮细胞在缺氧、再给氧及加入AS -ODN后ICAM - 1表达的阳性百分率。结果 :缺氧 10h ,肾小球血管内皮细胞ICAM - 1的表达与对照组无显著性差异 ,再给氧 6hICAM- 1的表达明显高于正常 ,加入AS -ODN后 ,ICAM - 1阳性细胞的百分率下降 40 6 %。结论 :AS -ODN可以降低H/R时内皮细胞ICAM - 1的表达
Objective: To investigate the effect of antisense oligonucleotide (AS-ODN) on the expression of intercellular adhesion molecule-1 (ICAM-1) in endothelial cells under hypoxia / reoxygenation (H / R) Methods: The positive percentage of ICAM - 1 in glomerular vascular endothelial cells after hypoxia, reoxygenation and AS-ODN was measured by flow cytometry. Results: After hypoxia for 10 hours, ICAM - 1 expression in glomerular vascular endothelial cells was not significantly different from that in control group. The expression of ICAM - 1 in reoxygenation 6hICAM - 1 was significantly higher than that in normal group. After adding AS - ODN, ICAM - 1 positive cells The percentage decreased by 40.6%. Conclusion: AS-ODN can reduce the expression of ICAM-1 in HUVECs