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目的:研究生姜水提物对全脑缺血再灌注大鼠脑组织氨基酸递质的影响。方法:SD雄性大鼠随机分为假手术组、模型对照组、尼莫地平组(30 mg.kg-1)、生姜高、中、低剂量组(200,100,50 mg.kg-1)。Pulsinelli’s四动脉阻断法造成全脑缺血再灌注损伤模型(CIR),分别于手术前1 d,术前1 h和再灌注前30 min ig给药,共3次。高效液相色谱法测定脑组织谷氨酸(Glu)、天冬氨酸(Asp)和甘氨酸(Gly)含量,原子吸收分光光度计测定脑组织Ca2+含量,干湿重法测定脑组织含水量,化学比色法测定脑组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:与假手术组比较,CIR模型组大鼠脑组织Glu,Ca2+,MDA含量和含水量明显升高(P<0.05或P<0.01),SOD活性明显降低(P<0.05);生姜水提物能显著降低脑组织Glu,Ca2+含量和含水量(P<0.05或P<0.01),显著升高SOD活性和SOD/MDA比值(P<0.05)。结论:生姜水提物防治脑缺血再灌注损伤的机制与降低兴奋性氨基酸(EAA)兴奋性毒性、阻滞Ca2+超载和提高抗氧化活性有关。
Objective: To study the effects of ginger extract on amino acid transmitters in brain tissue of rats with global cerebral ischemia and reperfusion. Methods: SD male rats were randomly divided into sham operation group, model control group, nimodipine group (30 mg.kg-1), high, medium and low dose ginger group (200,100,50 mg.kg-1). Pulsinelli’s four-artery occlusion method caused a global cerebral ischemia-reperfusion injury model (CIR) at 1 d before surgery, 1 h before surgery and 30 min before reperfusion. The contents of glutamic acid, aspartic acid and glycine in brain tissue were measured by HPLC. The contents of Ca2 + in brain tissue were measured by atomic absorption spectrophotometer. The contents of water in brain tissue were determined by dry and wet weight method. The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in brain tissue were measured by chemical colorimetry. Results: Compared with the sham operation group, the contents of Glu, Ca2 +, MDA and the content of water in the CIR model group were significantly increased (P <0.05 or P <0.01), and the SOD activity was significantly decreased (P <0.05) The contents of Glu, Ca2 + and water (P <0.05 or P <0.01) significantly increased the activity of SOD and the ratio of SOD / MDA in brain tissue (P <0.05). CONCLUSION: The mechanism of ginger extract in preventing and treating cerebral ischemia-reperfusion injury is related to reducing the excitotoxicity of excitatory amino acids (EAA), blocking the overload of Ca2 + and enhancing the anti-oxidative activity.