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目的:探讨Survivin在腺样囊性癌(ACC)细胞系中的表达情况及其表达与ACC远处转移的相关性。方法:培养人涎腺腺样囊性癌(ACC-2)和肺转移腺样囊性癌(ACC-M)细胞,观察其形态学特征,采用MTT法检测细胞的生长活性,并采用SP法检测细胞中Survivin的表达。结果:ACC-2和ACC-M细胞均表现有明显的上皮样和腺样细胞的特征。ACC-M细胞的生长活性高于ACC-2细胞,且Survivin在ACC-M细胞中的表达也强于ACC-2细胞。结论:Survivin与涎腺ACC肺部转移的发生具有高度相关性。
Objective: To investigate the expression of survivin in adenoid cystic carcinoma (ACC) cell lines and its correlation with the distant metastasis of ACC. Methods: The human salivary adenoid cystic carcinoma (ACC-2) and lung metastatic adenoid cystic carcinoma (ACC-M) cells were cultured and the morphological characteristics were observed. The cell growth activity was detected by MTT assay. Survivin expression in cells was measured. Results: Both ACC-2 and ACC-M cells showed obvious features of epithelial and adenoid cells. The growth activity of ACC-M cells was higher than that of ACC-2 cells, and the expression of Survivin in ACC-M cells was also stronger than that of ACC-2 cells. Conclusion: Survivin is highly correlated with the occurrence of ACC lung metastasis in salivary gland.