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为支持红松苗越冬伤害是一种光氧化伤害的新假说,本文提供了下述实验证据:1.全光苗在高光强下释放CO_2与吸收O_2相偶合,且不能被光呼吸抑制剂所抑制,这表明释放的CO_2不是来自光呼吸。2.全光苗针叶含有较高的不饱和脂肪酸过氧化物,而其叶绿体类囊体膜所含脂肪酸的不饱和度较低。3.对针叶起保护作用的SOD酶活性全光苗低于遮荫苗,一年生针叶低于二年生针叶,其同工酶谱在大树针叶上显示两条带而在幼苗针叶上只显示一条带。4.作为单线态氧猝灭剂的类胡萝卜素的含量也表现出遮荫苗高于全光苗,3月份尤为明显。
In order to support the overwinter injury of red pine seedlings is a new hypothesis of photooxidation damage, the following experimental evidence is provided in this paper: 1. All-light seedlings release CO2 at high light intensity and couple with absorbed O2, and can not be inhibited by photorespiratory inhibitors, This indicates that the released CO 2 does not come from photorespiration. 2. All seedlings needles contain higher unsaturated fatty acid peroxide, while the chloroplast thylakoid membrane contains fatty acids less unsaturated. The protective effect of the needle on the SOD activity of all-light seedlings is lower than the shade seedlings, annual conifer is lower than the biennial cones, the isozyme zymogram shows two bands in the coniferous seedlings Only show a band. 4. The content of carotenoids as a singlet oxygen quencher also showed that shading seedlings were higher than all seedlings, especially in March.