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目的:观察丁酸钠诱导培养的乳腺癌细胞MCF-7凋亡的影响。方法:用终浓度分别为0、1.25、2.5、5.0、10.0mmol/L的丁酸钠处理MCF-7细胞48h,用MTT比色法分析丁酸钠对细胞的抑制率,倒置显微镜观察细胞生长情况的改变,流式细胞术,末端脱氧核糖核酸转移酶介导的缺口末端标记法(TUNEL)及DNA琼脂糖凝胶电泳检测凋亡。结果:不同浓度的丁酸钠对MCF-7细胞有明显的抑制作用,光镜下观察到细胞贴壁性随作用时间延长而下降;流式细胞术检测不同浓度丁酸钠处理细胞的凋亡率分别为4.8%、9.4%、26.1%和51.9%;TUNEL检测可见阳性细胞胞体缩小,核固缩,呈棕黄色或黄褐色颗粒;DNA琼脂糖凝胶电泳检测到10mmol/L处理细胞出现“DNA ladders”。结论:丁酸钠可以诱导MCF-7细胞发生凋亡。
Objective: To observe the effect of sodium butyrate on the apoptosis of MCF-7 breast cancer cells. METHODS: MCF-7 cells were treated with sodium butyrate with final concentrations of 0, 1.25, 2.5, 5.0, and 10.0 mmol/L for 48 h. The inhibition rate of sodium butyrate on cells was analyzed by MTT colorimetry. Cell growth was observed under inverted microscope. Changes in conditions, flow cytometry, terminal deoxynucleotidase-mediated nick end labeling (TUNEL) and DNA agarose gel electrophoresis were used to detect apoptosis. RESULTS: Different concentrations of sodium butyrate had significant inhibitory effect on MCF-7 cells. Observed by light microscopy, cell adherence decreased with prolonged exposure time; Flow cytometry was used to detect apoptosis in cells treated with different concentrations of sodium butyrate. The rates were 4.8%, 9.4%, 26.1%, and 51.9%, respectively; TUNEL assay showed that the positive cells had reduced cell bodies, and the pyknosis was brown-yellow or yellow-brown particles; 10 mmol/L treated cells were detected by DNA agarose gel electrophoresis. DNA ladders”. Conclusion: Sodium butyrate can induce apoptosis in MCF-7 cells.