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目的:研究红花注射液致敏反应相关蛋白。方法:收集有/无致敏性红花注射液各3批,经离子交换树脂梯度洗脱富集其中的总蛋白,利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法分离蛋白并初步分析其差异。结果:40 k D和26 k D处,有致敏性红花注射液各有1个颜色较深、轮廓清晰的蛋白条带;10~17 k D处,无致敏性红花注射液有1个颜色较深、轮廓清晰的蛋白条带。结论:40 k D和26 k D处的蛋白可能是红花注射液致敏的原因之一,而17 k D处的蛋白可能不会引起过敏反应。致敏蛋白的分子结构及含量限度等仍需进一步研究。
Objective: To study saffron injection sensitized response protein. METHODS: Three batches of collected and non-sensitized safflower injection were collected and the total protein was eluted by ion exchange resin gradient. The protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) The proteins were separated and their differences were analyzed preliminarily. RESULTS: At 40 kD and 26 kD, sensitized safflower injection had one darker, clear-cut protein band; at 10-17 kD, the sensitized safflower injection had 1 A darker, clear outline of the protein band. CONCLUSIONS: Proteins at 40 kD and 26 kD may be responsible for the sensitization of safflower injection, whereas the protein at 17 kD may not cause an allergic reaction. Allergen protein molecular structure and content limits still need further study.