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Objective: To investigate U. parvum (previously Ureaplasma urealyticum biovar 1) and U. urealyticum (previously Ureaplasma urealyticum biovar 2) and their subtypes and serovars in urine specimens of pregnant women.Methods: After collecting 151 specimens and inoculating broth, all broth culture positive (urease positive) specimens were amplified, species were identified and subtyped by using general primers, species-specific, and type-specific primers targeting the multiple banded antigen (MBA) gene sequence.Results: U. parvum was identified in 58 of 151 specimens and U. urealyticum in 18 (both were present in 5, and neither were found in 6). Serovars 3, 1, and 6 were the most common among U. parvum isolates and subtypes 1 and 3 were the most common among U. urealyticum. Multiple serovars among clinical isolates were found.Conclusion: This PCR-based typing system could facilitate studies of the relationship between individual ureaplasma species or subtypes and human diseases.