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目的观察大鼠脑挫伤后脑组织NTE和COX-2的表达变化,并评价其法医学的意义。方法健康SD大鼠48只,随机分成正常对照组、手术对照组、挫伤组(按挫伤后处死时间再分为1h、12h、1d、3d、7d、14d共6个组)每组6只。以自由落体撞击法制作脑挫裂伤模型;应用免疫组化方法检测COX-2和NTE在脑组织中的表达情况;用SPSS 13.0软件分析COX-2和NTE的表达情况与脑挫伤后存活时间的相关性。结果正常对照组和假手术组NTE和COX-2均呈低水平表达,脑挫伤组伤后NTE和COX-2表达逐渐增强,均于1d达到高峰,至14d时COX-2表达降至正常对照组水平,而NTE仍高于正常对照组水平;挫伤各组与正常对照组比较,除1h组灰度值无统计学差异(P>0.05)外,其余各组均有明显差异(P<0.01)。结论脑挫伤后脑组织NTE和COX-2的表达均呈现先增强后下降的趋势,其表达规律有望为脑挫伤诊断及损伤时间推断提供参考。
Objective To observe the changes of NTE and COX-2 in brain tissue after traumatic brain injury in rats and to evaluate their significance in forensic science. Methods Forty-eight healthy SD rats were randomly divided into normal control group, operation control group and contusion group (6 rats in each group were divided into 6 groups at 1h, 12h, 1d, 3d, 7d, 14d after contusion). The expression of COX-2 and NTE in brain tissue were detected by immunohistochemical method. The expression of COX-2 and NTE in brain tissue were analyzed by SPSS 13.0 software and the survival time after cerebral contusion Relevance. Results The expression of NTE and COX-2 in normal control group and sham operation group showed low level. The expression of NTE and COX-2 in brain contusion group increased gradually after 1 week, reaching the peak at 1 day, and the expression of COX-2 decreased to normal level (P <0.01), while the NTE was still higher than that of the normal control group. There was a significant difference (P <0.01) between the contusion group and the normal control group except the gray value of 1h group was not significantly different (P> 0.05) ). Conclusions The expression of NTE and COX-2 in brain tissue of rats with brain contusion increased first and then decreased. The expression of NTE and COX-2 may be useful for the diagnosis of cerebral contusion and the estimation of injury time.