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目的:建立反相高效液相色谱法快速测定丹参中丹酚酸B、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA含量。方法:采用Kinetex核-壳技术色谱柱(100 mm×4.6 mm,2.6μm),以乙腈-0.1%三氟乙酸水溶液为流动相,梯度洗脱,柱温30℃,检测波长为280 nm(丹酚酸B)和254 nm(丹参酮类)。结果:丹酚酸B、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA5个成分浓度分别在3.40～850μg.mL-1(r=0.9998),0.96～240μg.mL-1(r=0.9999),2.04～255μg.mL-1(r=0.9995),0.27～68μg.mL-1(r=0.9998),1.12～210μg.mL-1(r=0.9997)范围内与峰面积呈现良好的线性;平均回收率(n=3)在97.32%～104.9%之间,RSD≤4.7%。结论:该方法快速、简便、灵敏,可用于丹参药材的质量控制。
Objective: To establish a reversed phase high performance liquid chromatography (HPLC) method for rapid determination of salvianolic acid B, dihydrotanshinone Ⅰ, cryptotanshinone, tanshinone Ⅰ and tanshinone Ⅱ A in Salvia miltiorrhiza. METHODS: Kinetex core-shell technique (100 mm × 4.6 mm, 2.6 μm) was used with a mobile phase of acetonitrile-0.1% trifluoroacetic acid as the mobile phase. The column temperature was 30 ℃ and the detection wavelength was 280 nm Phenolic acids B) and 254 nm (tanshinone). Results: The five components of salvianolic acid B, dihydrotanshinone Ⅰ, cryptotanshinone, tanshinone Ⅰ and tanshinone Ⅱ A were 3.40-850μg.mL-1 (r = 0.9998), 0.96-240μg.mL-1 (r = 0.9999) , 2.04-255μg.mL-1 (r = 0.9995), 0.27-68μg.mL-1 (r = 0.9998), 1.12-210μg.mL-1 (r = 0.9997) The recoveries (n = 3) ranged from 97.32% to 104.9% with RSD ≤ 4.7%. Conclusion: The method is rapid, simple and sensitive and can be used for the quality control of Radix Salviae Miltiorrhizae.