目的　探讨多癌基因反义寡核苷酸对慢性髓细胞白血病 (CML )的作用及其相互作用的影响 .方法　用台盼蓝拒染、集落形成、逆转录 -多聚酶链反应及流式细胞仪技术研究反义寡核苷酸对 K5 6 2细胞株和 CML 骨髓细胞的作用 .结果　 c- myb反义寡核苷酸通过促进 CML 细胞凋亡 ,发挥对 bcr/abl反义寡核苷酸的协同作用 .结论　多癌基因反义核酸的联合应用可为 CML基因治疗提供一项新手段 Objective To investigate the effect of multi-cancer antisense oligodeoxynucleotides on chronic myeloid leukemia (CML) and their interactions. Methods Trypan blue exclusion, colony formation, reverse transcription-polymerase chain reaction and flow cytometry Technical studies on the effects of antisense oligonucleotides on K562 cells and CML bone marrow cells. Results c-myb antisense oligonucleotides exert antisense oligonucleotides against bcr/abl by promoting apoptosis in CML cells. Synergistic effect. Conclusion The combination of antisense nucleic acids for cancer can provide a new approach for CML gene therapy.