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作者介绍一种新的固相酶免疫技术(EIA)以检测风疹病毒特异性IgG用辣根过氧化物酶(HRPO)标记半纯化风疹病毒抗原。半纯化抗原制备的方法是在Halone和Meurman法的基础上加以改进而来的:在700cm~2旋转瓶中,以含5%FCS、5%TPB、10μg/ml庆大霉素的199营养液培养Vero细胞4-5天后,弃去营养液,用BME(其中加入4%F-CS,5%TPB、1%精氨酸、10μg/ml庆大霉素)将约10~(-7)感染温度的Therien风疹病毒稀释至1:1000,以稀释的风疹病毒20ml加入各管,置35℃1.5小时
The authors describe a novel solid-phase enzyme immunoassay (EIA) for the detection of rubella virus-specific IgG-labeled semi-purified rubella antigens with horseradish peroxidase (HRPO). Semi-purified antigen preparation method is based on the Halone and Meurman method was improved from: In 700cm ~ 2 spinner flask with 5% FCS, 5% TPB, gentamycin 10μg / ml of 199 nutrient solution After culturing Vero cells for 4-5 days, the nutrient solution is discarded and about 10-7 (-7) cells are treated with BME (4% F-CS, 5% TPB, 1% arginine, 10 μg / ml gentamycin) Infected Therien rubella virus was diluted to 1: 1000, added with 20 ml of diluted rubella virus to each tube and set at 35 ° C for 1.5 hours