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为探究湛江近海域海洋沉积物中含氯多环芳烃的生物学毒性效应,选取湛江近海域2个地点(TS和JSW)采集样品,以含氯多环芳烃为目标,制备沉积物提取物,将斑马鱼胚胎胚胎暴露于不同浓度的沉积物提取物中,观测供试斑马鱼胚胎的死亡和畸形情况。结果表明:TS采样点沉积物提取物的LC50为15.98 mg/mL(48 h)和14.41mg/mL(96 h),JSW采样点沉积物提取物的LC50为19.89 mg/mL(48 h)和16.36 mg/mL(96 h),且致死效应呈现浓度依赖关系;TS和JSW 2个采样点沉积物中含氯多环芳烃能导致斑马鱼胚胎卵黄囊水肿、心包水肿、脊椎弯曲等致畸效应,其致畸效应与暴露浓度基本呈浓度依赖关系。湛江近海域沉积含氯多环芳烃物提取物对斑马鱼胚胎具有明显的致死作用和致畸毒性。
In order to explore the biological toxic effects of chlorine-containing polycyclic aromatic hydrocarbons (PAHs) in marine sediments near Zhanjiang, samples were collected from two locations in Zhanjiang offshore (TS and JSW) and chlorinated PAHs were used to prepare sediment extracts. The zebrafish embryo embryos were exposed to different concentrations of sediment extracts to observe the death and deformity of the tested zebrafish embryos. The results showed that the LC50 of sediment extract at TS sampling point was 15.98 mg / mL (48 h) and 14.41 mg / mL (96 h) respectively, and the LC50 of sediment extract at JSW sampling point was 19.89 mg / mL 16.36 mg / mL (96 h), and the lethal effect showed a concentration-dependent relationship. Chlorinated PAHs in the sediments of TS and JSW at two sampling sites could cause teratogenic effects such as yolk sac, pericardial edema and spinal curvature in zebrafish embryos , The teratogenic effect and exposure concentration in a concentration-dependent manner. The deposition of chlorine-containing polycyclic aromatic hydrocarbons (PAHs) extracts in Zhanjiang waters has significant lethal and teratogenic effects on zebrafish embryos.