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目的探讨5-HT1A受体激动剂丁螺环酮对大鼠局灶性脑挫裂伤(Controlled cortical impact injury,CCII)损伤周围区域神经凋亡的影响。方法 119只大鼠随机分为5组,A:空白对照组;B:空白对照+丁螺环酮组;C:假手术组;D:局灶性脑挫裂伤组+NS对照组;E:局灶性挫裂伤+丁螺环酮治疗组。参照改良Feeney’s自由落体硬膜外撞击法制作局灶性脑挫裂伤模型,伤后1小时予以腹腔注射丁螺环酮(0.3mg/Kg)或等体积生理盐水(Normal saline,NS),在规定时间点,灌注取脑、石蜡包埋、切片,HE染色观察损伤周围区域皮质病理表现;免疫组化检测损伤灶周围皮层脑组织Bax、Bcl-2及Caspase-3蛋白的表达;TUNEL法检测损伤皮层凋亡细胞。结果 A、B、C组各指标阳性表现很弱,各指标没有明显的差异(P>0.05);D、E组各时间点各指标与A、B、C组比较有明显差异(P<0.01);D、E组之间各时间点各指标比较也有明显的统计学意义(P<0.05或P<0.01)。结论丁螺环酮对大鼠局灶性脑损伤后损伤灶周围皮层的神经细胞凋亡具有抑制作用。
Objective To investigate the effect of buspirone, a 5-HT1A receptor agonist, on neuronal apoptosis in rats with focal cerebral contusion (CCII) injury. Methods 119 rats were randomly divided into 5 groups: A: blank control group; B: blank control + buspirone group; C: sham operation group; D: focal cerebral contusion group + NS control group; E : Focal contusion and buspirone treatment group. Focal cerebral contusion models were made according to the modified Feeney’s free-fall epidural method. Buspirone (0.3 mg / kg) or normal saline (NS) was injected intraperitoneally 1 hour after injury. At the prescribed time points, the cerebral cortex, paraffin embedded, sectioned and stained with HE were used to observe the pathological changes of cortex in the surrounding area. The expression of Bax, Bcl-2 and Caspase-3 protein in the cortex brain tissue around the lesion was detected by immunohistochemistry. Damaged cortical apoptotic cells. Results The positive expression of each index in groups A, B and C was weak, and there was no significant difference between each index (P> 0.05). There was significant difference between groups A, B and C at each time point (P <0.01) ); D, E groups at all time points between the various indicators also had significant statistical significance (P <0.05 or P <0.01). Conclusion Buspirone can inhibit the neuronal apoptosis in the cortex around the focal lesion in rats after focal brain injury.