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Objective:The aim of the study was to investigate whether enhanced anti-tumor effects of endostar(ES) on radiation involved hypoxia inducible factor-1α(HIF-1α) and basic fibroblast growth factor(bFGF).Methods:A549 cells were divided into four groups:control group;endostar(ES) group;radiation(RT) group;endostar plus radiation(ES + RT) group.The inhibition of proliferation rates of A549 cells was measured by cell counting kit-8(CCK-8).HIF-1α and bFGF expression levels were checked by enzyme linked immunosorbent assay(ELISA) and reverse transcription-polymerase chain reaction(RT-PCR).Results:The proliferation inhibition rate in the ES + RT group was higher than that in other groups.On the other hand,the expressions of HIF-1α and bFGF in the ES + RT group were significantly reduced compared to other groups.HIF-1α and bFGF levels were positively correlated(r=0.80,P<0.01).Conclusion:Our results suggest that endostar could enhance anti-tumor effect of radiation by reducing expressions of HIF-1α and bFGF.
Objective: The aim of the study was to investigate whether enhanced anti-tumor effects of endostar (ES) on radiation involved hypoxia inducible factor-1α (HIF-1α) and basic fibroblast growth factor (bFGF). Methods: A549 cells were divided into four groups: control group; endostar (ES) group; radiation (RT) group; endostar plus radiation (ES + RT) group. inhibition of proliferation rates of A549 cells was measured by cell counting kit- 8 (CCK-8). HIF-1α and bFGF expression levels were checked by enzyme linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). Results: The proliferation inhibition rate in the ES + RT group was higher than that in other groups. Hif-1α and bFGF levels were positively correlated (r = 0.80, P <0.01) .Conclusion: Our results suggest that endostar could enhance anti-tumor effect of radiation by reducing expressions of HIF-1α and bFGF.