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用转Cry1Ab/Cry1Ac基因水稻的叶片饲喂稻纵卷叶螟Cnaphalocrocis medinalis(Guene)幼虫,采用酶活力测定方法研究了转基因水稻对稻纵卷叶螟幼虫体内3种保护酶(SOD,CAT和POD)活性的影响。结果表明:取食转基因水稻叶片4h后,幼虫体内SOD酶活性比对照提高了91.5%,与对照有显著差异;36h后活性达到最大值,但与对照差异不显著。取食转基因水稻叶片24h后,幼虫体内CAT酶活性达到最大值且高于对照,但与对照差异不显著;48h后酶活性受到显著抑制,与对照差异显著。取食转基因水稻叶片12h后,幼虫体内POD酶活性达到最大值,与对照差异不显著;48h后酶活性逐渐下降达到最小值,比对照下降68.05%。实验同时测定了幼虫体内及其粪便中Bt毒蛋白含量的变化,结果表明取食转基因水稻叶片12h后,随着大量取食进入体内的毒蛋白随粪便排出,幼虫体内的毒蛋白含量一直低于粪便中的含量,且在24h时两者差异达到最大。由于Bt毒蛋白在幼虫体内的积累,扰乱了稻纵卷叶螟幼虫体内SOD,CAT和POD3种保护酶的动态平衡,使虫体内自由基的清除遇到障碍,从而对其产生毒害作用。
Cnaphalocrocis medinalis (Guenée) larvae were fed on the leaves of Cry1Ab / Cry1Ac transgenic rice plants. The activities of three protective enzymes (SOD, CAT And POD) activity. The results showed that the activities of SOD in the larvae increased by 91.5% compared with the control 4h after inoculation of transgenic rice leaves, which was significantly different from the control. The activity reached the maximum after 36 hours, but the difference was not significant with the control. The CAT activity in larvae reached the maximum value after 24h of transgenic rice leaves feeding, but the difference was not significant compared with the control. After 48 hours, the enzyme activity was significantly inhibited, which was significantly different from the control. After 12h of transgenic rice leaves, the activity of POD in larvae reached the maximum, which was not significantly different from the control. After 48h, the enzyme activity decreased to the minimum, which was 68.05% lower than the control. At the same time, the contents of Bt protein in larvae and their feces were measured. The results showed that after 12h of transgenic rice leaves, the content of toxic protein in larvae was always lower than Stool content, and at 24 h the difference between the two reached the maximum. Due to the accumulation of Bt toxin in larvae, it disturbed the dynamic balance of SOD, CAT and POD3 protective enzymes in the larvae of the rice leafroller larvae, which resulted in the poisoning of free radicals in the larvae.