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测定了N 磷酰化叉肽 ( 2 5)在溶液中的NMR谱 .发现它们具有非对映异位 ,分子无规卷曲及氢键相互作用 .波谱分析表明 ,磷酰基的参与 ,主要影响其结合部位的氨基酸 .由于肽平面的生成及分子内、分子间氢键影响的改变 ,致使叉肽中支链的Dipp Ala磷酰基与 β位羰基的相对位置发生变化 .磷酰基与N Cα 基团仍处于反式位置 ,这可能是该氨基酸侧链无极性基团的缘故
The NMR spectra of N-phosphorylated peptide (25) in solution were determined and found to have diastereomerism, molecular random coil and hydrogen bonding interactions.The spectral analysis showed that the involvement of phosphoryl group mainly affected its Amino acids at the binding site.Due to the changes of the peptide plane and the influence of intramolecular and intermolecular hydrogen bonds, the relative position of the branched-chain Dipp Ala phosphoryl group and the beta -carbonyl group in the peptide is changed.The combination of phosphoryl group and NCa group Still in the trans position, this may be due to the non-polar group of the amino acid side chain